double-positive cd21+cd27+ b cells are highly proliferating memory cells and their distribution differs in mucosal and peripheral tissuesdouble-positive cd21 + cd27 + b细胞是高度增殖表征记忆细胞及其分布在粘膜和外围组织有所不同.pdfVIP

Double-Positive CD21+CD27+ B Cells Are Highly Proliferating Memory Cells and Their Distribution Differs in Mucosal and Peripheral Tissues 1 2 2 3 2 2 Arpita Das , Huanbin Xu , Xiaolei Wang , Canddy L. Yau , Ronald S. Veazey , Bapi Pahar * 1 Division of Microbiology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America, 2 Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America, 3 Department of Biostatistics, School of Public Health and Tropical Medicine, Tulane University, New Orleans, Louisiana, United States of America Abstract Background: Several B-cell defects arise in HIV infected patients, particularly in patients with chronic infection and high viral load. Loss of memory B cells (CD27+ B cells) in peripheral blood and lymphoid tissues is one of the major B cell dysfunctions in HIV and simian immunodeficiency virus (SIV) infection. Despite several studies, definitive identification of memory B cells based on CD27 surface expression has not been described. Similarly, the rates of cell turnover in different B cell subpopulation from lymphoid and mucosal tissues have not been well documented. In this study, we demonstrate the presence of memory B cell populations and define their distribution, frequency and immunophenotype with regards to activation, proliferation, maturation, and antibody production in normal rhesus macaques from different lymphoid tissues. Methodology/Principal Findings: Thirteen healthy, uninfected rhesus macaques were selected for this study. CD20+ B cells were isolated from peripheral blood and so