ecdysone induced gene expression is associated with acetylation of histone h3 lysine 23 in drosophila melanogaster蜕皮激素诱导基因表达与赖氨酸乙酰化组蛋白h3的23日在黑腹果蝇.pdfVIP

Ecdysone Induced Gene Expression Is Associated with Acetylation of Histone H3 Lysine 23 in Drosophila melanogaster ´ ´ 1 ¤a ´ 1,2 ´ ´ ´ 1 ´ ´ 1¤b ´ 2,3 Laszlo Bodai * , Nora Zsindely , Renata Gaspar , Ildiko Kristo , Orban Komonyi , Imre ´ 1,2,4 Miklos Boros 1 Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary, 2 Chromatin Research Group, Hungarian Academy of Sciences, University of ´ ´ ´ Szeged, Szeged, Hungary, 3 Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS UMR 7104 - Inserm U 964, Illkirch, France, 4 Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Abstract Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limited or no correlation with ecdysone induced transcriptional activity. We found that dCBP, which is encoded by the nejire