efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, enterobacter cloacae subsp. dissolvens sdm高效的2,3-butanediol crop-biomass-utilizer生产从木薯粉,肠杆菌泄殖腔无性系种群。.pdfVIP

Efficient 2,3-Butanediol Production from Cassava Powder by a Crop-Biomass-Utilizer, Enterobacter cloacae subsp. dissolvens SDM 1. 1. 1 1 1 1 2 1,2 Ailong Wang , Youqiang Xu , Cuiqing Ma *, Chao Gao , Lixiang Li , Yu Wang , Fei Tao , Ping Xu 1 State Key Laboratory of Microbial Technology, Shandong University, Jinan, People’s Republic of China, 2 School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract Background: 2,3-Butanediol (BD) is considered as one of the key platform chemicals used in a variety of industrial applications. It is crucial to find an efficient sugar-utilizing strain and feasible carbon source for the economical production of BD. Methodology/Principal Findings: Efficient BD production by a newly isolated Enterobacter cloacae subsp. dissolvens SDM was studied using crop-biomass cassava powder as substrate. The culture conditions and fermentation medium for BD production were optimized. Under the optimal conditions, 78.3 g l21 of BD was produced after 24 h in simultaneous saccharification and fermentation (SSF), with a yield of 0.42 g BD g21 cassava powder and a specific productivity of 3.3 g l21 h21. A higher BD concentration (93.9 g l21) was produced after 47 h in fed-batch SSF. Conclusions/Significance: The results suggest that strain SDM is a good candidate for the BD production, and cassava powder could be used as an alternative substrate for the efficient production of BD. Citation: Wang A, Xu Y, Ma C, Gao C, Li L, et al. (2012) Efficient 2,3-Butanediol Production from Cassava Powder by a Crop-Biomass-Utilizer, Enterobacter