efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis有效的防污表面定量分析基于表面等离子体共振生物传感器.pdfVIP

Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis 1 ´ 1 2 1 1 Claude Nogues , Herve Leh , Joseph Lautru , Olivier Delelis , Malcolm Buckle * 1 LBPA, ENS de Cachan, CNRS, Cachan, France, 2 Institut d’Alembert, ENS de Cachan, Cachan, France Abstract Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non- specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated. Citation: Nogues C, Leh H, Lautru J, Delelis O, Buckle M (2012) Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis. PLoS ONE 7(9): e44287. doi:10.1371/journal.pone.0044287 Editor: Miklos S. Kellermayer, Semmelweis University, Hungary Received April 9, 2012; Accepted August 1, 2012; Published September 12, 2012 Copyright: 2012 Nogues et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.