electrophysiological and behavioral responses of male fall webworm moths (hyphantria cunea) to herbivory-induced mulberry (morus alba) leaf volatiles电生理和行为反应的男性美国白蛾飞蛾(hyphantria cunea),herbivory-induced桑桑属阿尔巴叶挥发物.pdfVIP

Electrophysiological and Behavioral Responses of Male Fall Webworm Moths (Hyphantria cunea) to Herbivory- Induced Mulberry (Morus alba) Leaf Volatiles 1,2 1 1 Rui Tang , Jin Ping Zhang , Zhong Ning Zhang * 1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People’s Republic of China, 2 Graduate University of the Chinese Academy of Sciences (GUCAS), Beijing, People’s Republic of China Abstract Volatile organic compounds (VOCs) were collected from damaged and intact mulberry leaves (Morus alba L., Moraceae) and from Hyphantria cunea larvae by headspace absorption with Super Q columns. We identified their constituents using gas chromatography-mass spectrometry, and evaluated the responses of male H. cunea antennae to the compounds using gas chromatography-flame ionization detection coupled with electroantennographic detection. Eleven VOC constituents were found to stimulate antennae of male H. cunea moths: b-ocimene, hexanal, cis-3-hexenal, limonene, trans-2-hexenal, cyclohexanone, cis-2-penten-1-ol, 6-methyl-5-hepten-2-one, 4-hydroxy-4-methyl-2-pentanone, trans-3-hexen-1-ol, and 2,4- dimethyl-3-pentanol. Nine of these chemicals were released by intact, mechanically-damaged, and herbivore-damaged leaves, while cis-2-penten-1-ol was released only by intact and mechanically-damaged leaves and b-ocimene was released only by herbivore-damaged leaves. Results from wind tunnel experiments conducted with volatile components indicated that male moths were significantly more attracted to herbivory-induced volatiles than the solvent control. Furthermore, male moths’ attraction to a sex pheromone lure was increased by herbivory-induced compounds and b-ocimene, but