electroporating fields target oxidatively damaged areas in the cell membraneelectroporating领域目标氧化细胞膜受损区域.pdfVIP

Electroporating Fields Target Oxidatively Damaged Areas in the Cell Membrane 1,2 2,3 4 5,6 2,4 P. Thomas Vernier *, Zachary A. Levine , Yu-Hsuan Wu , Vanessa Joubert , Matthew J. Ziegler , Lluis M. Mir5,6, D. Peter Tieleman7 1 Ming Hsieh Department of Electrical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States of America, 2 MOSIS, Information Sciences Institute, Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States of America, 3 Department of Physics and Astronomy, University of Southern California, Los Angeles, California, United States of America, 4 Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States of America, 5 Centre National de la Recherche ´ ´ ´ Scientifique, Unite Mixte de Recherche 8121–Vectorology and Gene Transfer, Institut Gustave Roussy, Villejuif, France, 6 Unite Mixte de Recherche 8121, Universite Paris- Sud, Orsay, France, 7 Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada Abstract Reversible electropermeabilization (electroporation) is widely used to facilitate the introduction of genetic material and pharmaceutical agents into living cells. Although considerable knowledge has been gained from the study of real and simulated model membranes in electric fields, efforts to optimize electroporation protocols are lim