elevated incidence of dental caries in a mouse model of cystic fibrosis龋齿的发病率上升在囊性纤维化小鼠模型.pdfVIP

Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis ´ 1¤ 2 2 2 2 Marcelo A. Catalan , Kathleen Scott-Anne , Marlise I. Klein , Hyun Koo , William H. Bowen , James E. 1 ¤ Melvin * 1 Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, United States of America, 2 Center for Oral Biology, University of Rochester Medical Center, Rochester, New York, United States of America Abstract Background: Dental caries is the single most prevalent and costly infectious disease worldwide, affecting more than 90% of the population in the U.S. The development of dental cavities requires the colonization of the tooth surface by acid- producing bacteria, such as Streptococcus mutans. Saliva bicarbonate constitutes the main buffering system which neutralizes the pH fall generated by the plaque bacteria during sugar metabolism. We found that the saliva pH is severely decreased in a mouse model of cystic fibrosis disease (CF). Given the close relationship between pH and caries development, we hypothesized that caries incidence might be elevated in the mouse CF model. Methodology/Principal Findings: We induced carious lesions in CF and wildtype mice by infecting their oral cavity with S. mutans, a well-studied cariogenic bacterium. After infection, the mice were fed a high-sucrose diet for 5 weeks (diet 2000). The mice were then euthanized and their jaws removed for caries scoring and bacterial counting. A dramatic increase in caries and severity of lesions scores were apparent in CF mice compared to their wildtype littermates. The elevated incidence of carious