elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiologynon-esterified脂肪酸浓度升高在牛卵母细胞成熟早期胚胎生理妥协.pdfVIP

Elevated Non-Esterified Fatty Acid Concentrations during Bovine Oocyte Maturation Compromise Early Embryo Physiology 1 2 3 3 Veerle Van Hoeck *, Roger G. Sturmey , Pablo Bermejo-Alvarez , Dimitrios Rizos , Alfonso Gutierrez- 3 2 1 1 Adan , Henry J. Leese , Peter E. J. Bols , Jo L. M. R. Leroy 1 Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of ´ ´ Antwerp, Wilrijk, Belgium, 2 Hull-York Medical School, University of Hull, Hull, United Kingdom, 3 Departamento de Reproduccion Animal y Conservacion de Recursos ´ Zoogeneticos, INIA, Madrid, Spain Abstract Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturation affect the development and physiology of zygotes formed from such oocytes, using the cow as a model. The zygotes were grown to blastocysts, which were evaluated for their quality in terms of cell number, apoptosis, expression of key genes, amino acid turnover and oxidative metabolism. Oocyte maturation under elevated NEFA concentrations resulted in blastocysts with significantly lower cell number, increased apoptotic cell ratio and altered mRNA abundance of DNMT3A,