elevated levels of cell-free circulating dna in patients with acute dengue virus infection中循环游离dna水平升高的患者急性登革热病毒感染.pdfVIP

Elevated Levels of Cell-Free Circulating DNA in Patients with Acute Dengue Virus Infection 1 1 2 3 4 Tran Thi Ngoc Ha , Nguyen Tien Huy , Lyre Anni Murao , Nguyen Thi Phuong Lan , Tran Thi Thuy , Ha 4 5 5 5 1 1 Manh Tuan , Cao Thi Phi Nga , Vo Van Tuong , Tran Van Dat , Mihoko Kikuchi , Michio Yasunami , 2 3 1,6 Kouichi Morita , Vu Thi Que Huong , Kenji Hirayama * 1 Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan, 2 Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan, 3 Laboratory of Arbovirus, Pasteur Institute, Ho Chi Minh City, Vietnam, 4 Children’s Hospital No. 2, Ho Chi Minh City, Vietnam, 5 Center for Preventive Medicine, Vinh Long, Vietnam, 6 Global COE program, Nagasaki University, Nagasaki, Japan Abstract Background: Apoptosis is thought to play a role in the pathogenesis of severe dengue and the release of cell-free DNA into the circulatory system in several medical conditions. Therefore, we investigated circulating DNA as a potential biomarker for severe dengue. Methods and Findings: A direct fluorometric degradation assay using PicoGreen was performed to quantify cell-free DNA from patient plasma. Circulating DNA levels were significantly higher in patients with dengue virus infection than with other febrile illnesses and healthy controls. Remarkably, the increase of DNA levels correlated with the severity of dengue. Additionally, multivariate logistic