notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice血统和肠道干细胞的活动由一组新的敲入小鼠.pdfVIP

Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice 1 2 3 1 4 5 Silvia Fre , Edouard Hannezo , Sanja Sale , Mathilde Huyghe , Daniel Lafkas , Holger Kissel , Angeliki 6 7 1 3,4,8 Louvi , Jeffrey Greve , Daniel Louvard , Spyros Artavanis-Tsakonas * 1 Morphogenesis and Intracellular Signaling, Institut Curie-UMR144 CNRS, Paris, France, 2 Physical Chemistry Curie, Institut Curie-UMR 168 CNRS-UPMC, Paris, France, 3 Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America, 4 Department of Developmental Biology and Genetics, Institut ¨ Curie-UMR3215 CNRS-U934 Inserm, Paris, France, 5 TaconicArtemis GmbH, Koln, Germany, 6 Program on Neurogenetics and Departments of Neurosurgery and Neurobiology, Yale School of Medicine, New Haven, Connecticut, United States of America, 7 Exelixis, Inc., South San Francisco, California, United States of America, ` 8 College de France, Paris, France Abstract The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that