novel cell and tissue acquisition system (ctas) microdissection of live and frozen brain tissues新细胞和组织采集系统(cta)显微解剖的生活和冰冻的脑组织.pdfVIP

Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues 1 1 1,2 3 4 4 Lili C. Kudo *, Nancy Vi , Zhongcai Ma , Tony Fields , Nuraly K. Avliyakulov , Michael J. Haykinson , 1,3 1 Anatol Bragin , Stanislav L. Karsten * 1 NeuroInDx, Inc., Signal Hill, California, United States of America, 2 Division of Neuroscience, Department of Neurology, Los Angeles Biomedical Research Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, United States of America, 3 Department of Neurology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America, 4 Department of Biological Chemistry, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America Abstract We developed a novel, highly accurate, capillary based vacuum-assisted microdissection device CTAS - Cell and Tissue Acquisition System, for efficient isolation of enriched cell populations from live and freshly frozen tissues, which can be successfully used in a variety of molecular studies, including genomics and proteomics. Specific diameter of the disposable capillary unit (DCU) and precisely regulated short vacuum impulse ensure collection of the desired tissue regions and even individual cells. We demonstrated that CTAS is capable of dissecting specific regions of live and frozen mouse and rat brain tissues at the cellular resolution with high accuracy. CTAS based microdissection avoids potentially harmful physical treatment of