novel insights into the transcriptome of dirofilaria immitis新颖的见解的转录组dirofilaria巨细胞.pdfVIP

Novel Insights into the Transcriptome of Dirofilaria immitis 1. 2. 2 2 1 1 1 Yan Fu , Jingchao Lan , Zhihe Zhang , Rong Hou , Xuhang Wu , Deying Yang , Runhui Zhang , 1 1 1 1 2 2 2 2 Wanpeng Zheng , Huaming Nie , Yue Xie , Ning Yan , Zhi Yang , Chengdong Wang , Li Luo , Li Liu , 1 1 3 1 Xiaobin Gu , Shuxian Wang , Xuerong Peng , Guangyou Yang * 1 Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China, 2 The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife – Developing toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China, 3 Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an, China Abstract Background: The heartworm Dirofilaria immitis is the causal agent of cardiopulmonary dirofilariosis in dogs and cats, and also infects a wide range of wild mammals as well as humans. One bottleneck for the design of fundamentally new intervention and management strategies against D. immitis may be the currently limited knowledge of fundamental molecular aspects of D. immitis. Methodology/Principal Findings: A next-generation sequencing platform combining computational approaches was employed to assess a global view of the heartworm transcriptome. A total of 20,810 unigenes (mean length = 1,270 bp) were assembled from 22