specific far infrared spectroscopic properties of phospholipids特定的磷脂的远红外光谱特性.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 525–532 doi:10.1155/2012/279650 Specific Far Infrared Spectroscopic Properties of Phospholipids Ruth Hielscher1, 2 and Petra Hellwig1 1 Laboratoire de Spectroscopie Vibrationnelle et Electrochemie des Biomolecules, Institut de Chimie, ´ Universite de Strasbourg, UMR 7177, 1 rue Blaise Pascal, 67000 Strasbourg Cedex, France ´ 2 Strahlentherapie und Radioonkologie, Universitat Gottingen, Roberto Koch Strasse 40, ¨ ¨ 37075 Gottingen, Germany ¨ Correspondence should be addressed to Petra Hellwig, hellwig@unistra.fr Copyright © 2012 R. Hielscher and P. Hellwig. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. We describe the specific spectral signature of different phospholipids and sphingolipids in the far infrared. Three specific spectral domains have been found: the head group contributions (600 and 480 cm−1); the modes of the torsion motion of the hydrocarbon chains and of the skeleton vibration (460 to 180 cm−1); and the hydrogen-bonding continuum (below 300 cm−1). Marker bands for individual phospholipids are distinguished. Keywords: Bound water, far infrared, hydrogen bonds, self-organization, phospholipids 1. Introduction In natural membranes, a specific mixture of lipids is typically found for each organism [ 1]. The cell membranes of eukaryotes essentially consist of three classes of lipids: phospholipids, sphingolipids, and cholesterol. Inositol phospholipids are ubiquitous membrane lipids found in mammals as well as in bacteria and other organisms