the crude extract from puerariae flower exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice从puerariae花粗提物对各类典型,在高脂肪食源性肥胖老鼠antifatty肝脏的影响.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 272710, 6 pages doi:10.1155/2012/272710 Research Article The Crude Extract from Puerariae Flower Exerts Antiobesity and Antifatty Liver Effects in High-Fat Diet-Induced Obese Mice Tomoyasu Kamiya,1 Mayu Sameshima-Kamiya,1 Rika Nagamine,1 Masahito Tsubata,1 Motoya Ikeguchi,1 Kinya Takagaki,1 Tsutomu Shimada,2 and Masaki Aburada2 1 Research and Development Division, Toyo Shinyaku Co. Ltd., 7-28 Yayoigaoka, Tosu-shi, Saga 841-0005, Japan 2 Research Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo-Shi, Tokyo 202-8585, Japan Correspondence should be addressed to Tomoyasu Kamiya, kamiyat@toyoshinyaku.co.jp Received 17 January 2012; Accepted 28 March 2012 Academic Editor: Mohamed Eddouks Copyright © 2012 Tomoyasu Kamiya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Kudzu, a leguminous plant, has long been used in folk medicine. In particular, its flowers are used in Japanese and Chinese folk medicine for treating hangovers. We focused on the flower of Kudzu (Puerariae thomsonii), and we previously reported the antiobesity effect of Puerariae thomsonii flower extract (PFE) in humans. In this study, we conducted an animal study to investi- gate the effect of PFE on visceral fat and hepatic lipid levels in mice with diet-induced obesity. In addition, we focused on gene expression profiles to investigate the antiobesity mechanism of PFE. Male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with 5% PFE for 14 days. PFE supplementation significantly reduced body weight