the phthalocyanines applications in photodynamic therapy investigated by time-resolved and steady-state photothermal methods酞菁应用在光动力治疗调查时间分辨和稳态光热光谱分析方法.pdfVIP

Vol. 4 INTERNATIONAL JOURNAL OF PHOTOENERGY 2002 The phthalocyanines applications in photodynamic therapy investigated by time-resolved and steady-state photothermal methods Danuta Frackowiak,1 Krzysztof Wiktorowicz,2 Alfons Planner,1 Aneta Waszkowiak,1 and Rodica-Mariana Ion3 1 ´ ´ Faculty of Technical Physics, Poznan University of Technology, Nieszawska 13A, 60-965 Poznan, Poland 2 Department of Biology and Enviromental Studies, K. Marcinkowski University of Medical Sciences, ´ Długa 1/2, 61-848 Poznan, Poland 3 ZECASIN Splaiul Independentei 202, 79611 Bucharest, Romania Abstract. The time-resolved photothermal methods are used in order to establish the yield of intersystem (singlet-triplet) crossing of dyes that are candidates for the application in photodynamic therapy. It is known that molecules with long living triplet state cause efficient photosensitization by direct interaction with the cells or by singlet oxygen generation. The steady state photoacoustic spectra enables one to establish the dye incorporation into cells. Both methods were applied to several phthalocyanines and the results gathered by photothermal measure- ments were compared with the results obtained by means of other methods.The six investigated dyes were compared. 1. INTRODUCTION Dye molecule Oxygen molecule Phthalocyanines (Pcs) ar