the protective effect of apamin on lpsfat-induced atherosclerotic mice在lpsfat-induced apamin动脉粥样硬化小鼠的保护作用.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 305454, 10 pages doi:10.1155/2012/305454 Research Article The Protective Effect of Apamin on LPS/Fat-Induced Atherosclerotic Mice Soo-Jung Kim,1 Ji-Hyun Park,1 Kyung-Hyun Kim,1 Woo-Ram Lee,1 Sok Cheon Pak,2 Sang-Mi Han,3 and Kwan-Kyu Park1 1 Department of Pathology, School of Medicine, Catholic University of Daegu, 3056-6 Daemyung 4-Dong, Nam-Gu, Daegu 705-718, Republic of Korea 2 School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW 2795, Australia 3 Department of Agricultural Biology, National Academy of Agricultural Science, Suwon 441-100, Republic of Korea Correspondence should be addressed to Kwan-Kyu Park, kkpark@cu.ac.kr Received 10 October 2011; Revised 30 January 2012; Accepted 5 February 2012 Academic Editor: Myeong Soo Lee Copyright © 2012 Soo-Jung Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Apamin, a peptide component of bee venom (BV), has anti-inflammatory properties. However, the molecular mechanisms by which apamin prevents atherosclerosis are not fully understood. We examined the effect of apamin on atherosclerotic mice. Atherosclerotic mice received intraperitoneal (ip) injections of lipopolysaccharide (LPS, 2 mg/kg) to induce atherosclerotic change and were fed an atherogenic diet for 12 weeks. Apamin (0.05 mg/kg) was administered by ip injection. LPS-induced THP-1-derived macrophage inflammation treated with apamin reduced expression of tumor necrosis factor (TNF)-α, vascular cell adhesion molecule (VCAM)-1, and intracel