内皮祖细胞在胶质瘤新生血管中的募集和整合作用.doc

内皮祖细胞在胶质瘤新生血管中的募集和整合作用 徐承平 张华蓉 陈飞兰 章容 卞修武( （第三军医大学西南医院病理学研究所，重庆 400038） 摘 要：目的 探讨内皮祖细胞（endothelial progenitor cells，EPCs）在胶质瘤血管新生化过程的作用。方法 采用密度梯度离心法分离人脐血EPCs。应用人脑胶质瘤细胞系U87细胞建立原位移植动物模型，于瘤细胞接种后第7d将EPCs经尾静脉注入荷瘤鼠，并分别于尾静脉注射后第7、14和21d，观察EPCs植入后在移植瘤血管新生过程中的募集、整合作用以及对肿瘤生长的影响。结果 胶质瘤原位移植模型的成瘤率100%。在EPCs植入后的第14和21d，被募集和整合到肿瘤血管中的EPCs数量显著多于第7d(P0.05)，肿瘤的微血管密度和移植瘤的体积也显著高于对照组(P0.05)。结论 人脐血EPC能够被募集并整合到肿瘤血管中促进胶质瘤的生长，提示其在胶质瘤的血管新生化过程中可能具有重要作用。 关键词：内皮祖细胞；胶质瘤；血管新生化；肿瘤移植 中图法分类号：R733.3；R329.2+3 文献标识码： A Incorporation and recruitment of endothelial progenitor cells from human umbilical cord blood in glioma neovascularization XU Cheng-ping,ZHANG Hua-rong, CHEN Fei-lan, ZHANG Rong,BIAN Xiu-wu(Institute of Pathology, SouthwestHospital, Third Military Medical University, Chongqing 400038, China) Abstract: Objective To investigate the effects of endothelial progenitor cells derived from human umbilical cord blood in glioma neovascularization in vivo. Methods EPCs were isolated from human umbilical cord blood by density gradient centrifugation. U87 glioblastoma cells were implanted into the brain of nude mice using a stereotactic technique for replicating an orthotopically implanted human glioblastoma model. On 7 days after U87 cell implantation, EPCs were injected into tumor-bearing mice via their tail veins.Incorporation and recruitment of endothelial progenitor cells in neovascularization of malignant glioma in vivo were examined respectively by confocal laser scanning microscopy on days 7,14,21 after EPCs transplantation,and the expansion of the tumor mass were observed. Results Gliomas were formed in all the injected nude mice. The number of EPCs incorporated and recruited into newly formed tumor vessels at days 14,21 after EPCs transplantation were significantly exceeded than that at days 7 (P0.05).Moreover, the volume and vascular density of U87 xenograft were significantly increased in EPCs groups than in control groups(P0.05).Conclusion EPCs derived from human u