糖皮质激素抑制骨骼纵向生长的机制（Mechanism of glucocorticoid inhibiting longitudinal growth of bone）.docVIP

糖皮质激素抑制骨骼纵向生长的机制（Mechanism of glucocorticoid inhibiting longitudinal growth of bone） Mechanism of glucocorticoid inhibiting longitudinal growth of bone Abstract: Objective To study the mechanism of glucocorticoid inhibiting the longitudinal growth of bone through the study of growth plate, and to provide theoretical support for clinical prevention and treatment of growth retardation caused by glucocorticoids. Methods 20 clean male SD rats of 3 weeks old were used. The rats were randomly divided into dexamethasone group 12 and control group 8. The rats in the dexamethasone group were injected intraperitoneally with dexamethasone 200 g/100g body weight, and the control group was injected with volume physiological saline for 10 days. Weigh daily. The venous blood was collected on the day of slaughter, and the length of the nose and the length of the tibia were measured. Tissue sections of tibial growth plates were prepared. 1, to reflect the long tibial longitudinal bone growth, learn special staining method by Masson trichrome staining, Mallory staining and tissue morphology, display plate chondrocytes, direct measurement of growth plate thickness, the proliferation zone and hypertrophic zone thickness. 2. Immunohistochemical technique was used to observe the expression of glucocorticoid receptor, signal molecule TGF-B1 and Ihh in growth plate. 3, through the determination of serum concentration of ELISA, investigate the effect of dexamethasone treatment on the GH-IGF-I axis component, a marker of bone formation and bone calcium protein, alkaline phosphatase and type I collagen carboxy terminal propeptide and influence bone growth parameters of C type natriuretic peptide. SSPSS14.0 statistical software deals with data. The results were expressed by X + s. The independent sample t test, P 0.05, was significant difference. The results of the third day, rats in the dexamethasone group daily weight net growth was significantly lower than the control group rats; the sixth