不同碳源对解磷真菌溶解低品位磷矿能力的影响.pdfVIP

生物技术通报 # # BIO TE CHN OLOG Y B ULLETIN 20 11 5 1, 2 2 李露莉 邱树毅 1 2 ( , 550025; , 550025) : 以磷酸三钙作为唯 一磷源, 从磷矿区筛选出一株高效浸磷真菌经过核 体间隔区( ITS) 扩增序列分析, 确 定该菌株属于黑曲霉研究不同碳源条件下该菌株对低品位磷矿中磷浸出能力的影响; 此外, 还利用高效液相色谱法 ( H PLC)和耦合等离子体- 发射光谱仪对溶磷机理进行了初探结果表明, 以可溶性淀粉作为碳源的条件下, 真菌对低品 位磷矿的磷浸出率最高, 可能与其代谢产生的草酸和苹果酸及其有机酸总量有关, 同时, 磷浸出率与培养介质 pH 值呈显著 负相关 : 磷矿 浸磷真菌 磷浸出率 溶磷机理 Effect of D ifferent Carbon Sources on the Solubilization of Low Grade PhosphateM ine by Phosphate Solubilizing Fungi 1, 2 2 L i Luli Q iu Shuyi 1 2 ( College of Lfi e Science, Guizhou University, Guiyang 550025; Guizhou ProvincialK ey Laboratory of Fermentation Engineering an B iological Pharmacy, Guizhou University, Guiyang 550025) Abstract: A sperg illus niger iso la ted f om phosphate m ine wh ich is capab le of supe io leach ing phospho us, w as identified by the inte na l t ansc ibed space ana lys is. W e stud ied the effects of d iffe en t ca bon sou ces on the leaching of low g ade phospha te, and ex- p lo ed the m echan ism of ock phosphates d isso lved by A. niger w ith the app lication of HPLC and ICP-A ES. T he esu lts suggest that phospho us leaching a te is the h ighest by A. niger unde the cond ition of so luble sta ch compa ed to the othe ca bon sou ces, wh ich m ay be e la tive to ox alic ac id, m alic ac id and its to tal content. A t the sam e tmi e, nega tive elationship is betw een phosphate leach ing ca- pacity and pH of cu ltu e med ium s in d iffe ent ca bon sou ces K ey w ords: Pho sphate m ine L each ing phospho us fung i P hospho us leaching a te P