不同水系中华绒螯蟹线粒体co基因片段的rflp分析.pdfVIP

华北农学报2007, 22( 1) :78- 82 CO RFLP 1 1 1 1 1 2 王晓梅, 邢克智 , 戴 伟, 王 茜, 曹维维 , 李晓东 ( 1. , 300384; 2. , 124200) :PCR-RFLP 3 CO , 11 CO ,6 , 5 , 2, 3DNA 0006 7() 0016 7() 0.000 3() ,, , 3 , : , 0023 7,0020 7, 0004 3, 3 , : ; ; ; :S932.5+ 2 :A : 1000- 7091(2007) 01- 0078- 05 RFLP Analysis on Mitochondrial Cytochrome Oxidase I Gene of Chinese Mitten Crab (E . sinensis ) 1 1 1 1 1 2 WANG iao-mei , ING Ke-zhi , DAI Wei , WANG Qian , CAO Wei-wei , LI iao-dong ( 1.Tianjin Key laboratory of Aqua-Ecology and Aquaculture, Department of Fisheries Science, Tianjin Agricultural University, Tianjin 300384,China; 2. Panjin Guanghe Fisheries Company of Limited, Panjin 124200,China) Abstract:The fragments of mitochondrial cytochrome oxidase I of Eriocheir sinensis from Changjiang,Oujiang and Liaohewere amplified by using PCR-RFLP technique. 11 restriction endonucleases were used to digest the PCR prod- ucts. In this study therewere 6 compositehaplotypes in the 3 populations, therewere 5 compositehaplotypes in Oujiang population and 2 ones in population of Liaohe and Changjiang. The index of nucleotide diversity (value) were0.016 7 (Oujiang) , 0.006 7(Changjiang) and 0. 000 3 (Liaohe) respectively, indicating that the genetic diversity of Oujiang population was highest among the 3 populations, the genetic diversity of Changjiang population was higher than that of Liaohe population. Interpopulational net genetic distancewas thehighest (0.023 7) between Liaohe and Oujiang, while it was 0.020 7 between Changjiang and Oujiang andwas 0.0043 between Chanjiang andLiaohe samples. Theresults in-