7721 cell apoptosis in vitro cisplatin-induced liver cancer in SMMC cellular signal transduction factor activity change（7721细胞体外凋亡cisplatin-induced肝癌SMMC细胞信号转导因子活动的变化）.docVIP

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2017-09-05 发布于浙江
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7721 cell apoptosis in vitro cisplatin-induced liver cancer in SMMC cellular signal transduction factor activity change（7721细胞体外凋亡cisplatin-induced肝癌SMMC细胞信号转导因子活动的变化）.doc

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7721 cell apoptosis in vitro cisplatin-induced liver cancer in SMMC cellular signal transduction factor activity change（7721细胞体外凋亡cisplatin-induced肝癌SMMC细胞信号转导因子活动的变化）

7721 cell apoptosis in vitro cisplatin-induced liver cancer in SMMC cellular signal transduction factor activity change Author: Zheng Liang Keming of azelaic hung Different Growing [Keywords] apoptosis Keywords: mitosis the original activated protein kinase family; signal transduction; apoptosis; cisplatin; hepatoma SMMC-7721 cell lines; immunocytochemistry; flow cytometry Abstract: The purpose of in vitro use of SMMC-7721 cells apoptosis induced by cisplatin detect cell transduction factor MAPKs and transcription factors (JUN, CREB activity changes. Methods using immunocytochemistry and flow cytometry results 15mg L- a the cisplatin role of SMMC-7721 cultured cells 48h can be observed obvious apoptosis peak, 72h immunocytochemical staining found in the cytoplasm or (and nucleus of MAPKs (JIN / SAPKs p38MAPK, ERK and transcription factors (JUN, CREB significantly expressed. conclusion in the process of in vitro cisplatin-induced apoptosis of hepatoma SMMC-7721, MAPKs pathway (JIN / SAPKs, p38MAPK, ERK and transcription factors (JUN, CREB involved in the induction of apoptosis. Keywords: MAPKs; cisplatin; apoptosis; cell signal trans-duction; hepatoma SMMC-7721cell; FCM; im-munocytochemistry Abstract: AIM To investigate activity changes of cell signal transduction factors (JIN / SAPKs, p38MAPK, ERK, JUN, CREBin apoptotic hepatoma SMMC-7721cell induced by CDDP.METHODS Using immunocytochemistry and FCM methods.RESULTS After treatment with CDDP at15mg L-1, there were apoptotic peaks in cell cycle analysis on FCM for48h and expression of MAPKs and transcripton factors in cell plasm and / or nucleus for72h.CONCLUSION MAPKs pathway and transcription factors (JUN, CREBare con-cerned with apoptosis of hepatoma SMMC-7721cell induced by cisplatin . 0 Introduction In a multi-cell apoptosis, differentiation of animal development process has played a very important role [1] Although apoptosis in some cases referred to as cell suicide, but in most cases requ