nmr analysis of the intracellular free ions and ion channels（核磁共振分析细胞内自由离子和离子通道）.docVIP

NMR Analysis of the intracellular free ions and ion channels [Abstract] play an important role for the free ions in the cell metabolism and ion channel studies, determination of biological cells within the PH value. The PH cells are also part of the activity of the enzyme has a close relationship of exogenous gene transfection expression analysis of the PH in this type of cell commonly used monkey kidney cells, cos-7 to do experimental testing, provide a reliable basis for gene transfection. In this study, the 31P NMR method on the PH value of the cos-7 cells were measured and analyzed. [Keywords:] 31P NMR, cos-7 cell pH 1 Introduction Intracellular pH has always been the focus of biochemical and biological research is to study the most fundamental part is the intracellular and extracellular pH ion gradient, the ions in the intracellular pH under the influence of internal and external transformation, resulting in ion channels, affect cell function and metabolism of [1]. so intracellular PH and ion channels of the analysis of research, a number of physiological development and pathological change play an important role. In order to study the free ions of Ca2 + of Li + of Mg2 + and so on and channel the changes have created a lot of new bioanalytical methods. these kinds of analytical methods with continuous dynamic, long, injury-free advantage of new research methods for the observation of cell metabolism, energy conversion and pathological physiological changes because the cells function and metabolism by the influence of intracellular pH, so part of the activity of the enzyme and its closely linked to cellular metabolism and physiological data transformation, determined by intracellular PH method is the determination of intracellular PH weak base weak acid distribution method and Pt / H electrode method, the disadvantage is that the value is not accurate, and can not be repeated testing is currently widely used is PH sensitive electrode method, the mea