P110δ阻滞剂对哮喘小鼠NF-KB及IL-17表达的影响.pdfVIP

pathogenesis and multual relationship, and thus provide a theoretical basis for a new approach to asthma treatment.Methods:40 Balb/c mice were randomly divided into asthma group (X), azithromycin intervention group (A), blank control group (K), the IC87114 high-dose intervention group (Y1), IC87114 small-dose intervention group (Y2). The asthma group:each mouse was sensitized intraperitoneal injection of 0.2ml antigen suspension allergens (chicken ovalbumin OVA 100ug, aluminum hydroxide 2 mg, saline 0.2m1)in the first day, the 8th day, the 15th day by . To inspire by intranasally instill 40ulOVA solution in each mice after 0.75 ％ pentobarbital anesthesia in the 16th day and repeated it for 7days. Azithromycin intervention group :each mice sensitized as the asthma group, but was teated with intraperitoneal injection of azithromycin (50mg/kg)30 minutes before inspired .The IC87114 intervention group：each mice sensitized as the asthma group，but was given inhibitor at the 16th day ,the 18th day 1 hour before inspired and at the 21st day 3 hours after inspired .The high-dose and the small-dose were 1mg/kg.d, 0.1mg/kg.d. Blank control group: each mice was sensitized intraperitoneal injection of 0.2ml saline on the first day,the 8th day,the 15th day.Then they were inspired with saline for 7days. All mice were sacrificed after the last stimulation in 48 hours . Cut lung tissue and HE staining to evaluate the organizations general morphology and cel l infiltration; measure the content of IL-17, PIP3 in serum by enzyme- linked immunosorbent assay (ELISA) ; detect the expression of NF-KBp65 in lung tissue using immunohistochemistry ; test IL-17mRNA amount in lung tissue of mice by RT-PCR .All data was express with （ ）One-way ANOVA of variance was used in the study. X S pairwise comparisons using the SNK test, if the heterogeneity of variance Tamhanei France. Analysis the linear correlation of IL-17and IC87114