扁平苔藓皮损中MIF、MMP-9的表达和意义.pdfVIP

山西医科大学硕士学位论文 中文摘要 目的：研究巨噬细胞移动抑制因子（MIF ）与基质金属蛋白酶9 （MMP-9 ）在扁平苔 藓皮损中的表达及相互关系，进一步探讨两者在扁平苔藓发生发展中的作用及意义。 方法：采用免疫组织化学二步法和逆转录聚合酶链式反应（RT-PCR ）法分别检测30 例扁平苔藓组织和 30 例正常皮肤组织中 MIF 和 MMP-9 的表达情况。 结果：MIF 、MMP-9在扁平苔藓组织中有阳性表达，而在正常皮肤组织中呈阴性或弱 阳性表达。MIF 、MMP-9在扁平苔藓组织中的表达明显高于正常皮肤组织，差异有统计学 意义（P ＜0.05 ）；MIF和MMP-9在扁平苔藓皮损中的表达呈正相关（r=0.636 ，P ＜0.01 ）。 结论：（1）MIF可能参与了扁平苔藓炎细胞的浸润。（2 ）MMP-9可能在扁平苔藓基底 膜带的破坏中发挥了作用。（3 ）MIF 、MMP-9在扁平苔藓中的表达呈正相关，表明两者可 能通过相互作用参与了扁平苔藓基底膜带的破坏。 关键词：扁平苔藓；MIF ；MMP-9 ；基底膜带 I 山西医科大学硕士学位论文 英文摘要 The expression and significance of MIF and MMP-9 in lichen planus Abstract Objective: To detect the expression and relationship of MIF and MMP-9 in lichen planus.To investigate effect on the pathogenesis of lichen planus. Methods:Two-step Immunchistochemical technique and reverse transcriptase –polym erase chain reaction (RT-PCR) were used to test the expression of MIF and MMP-9 in 30 cases of lichen planus and 30 cases of normal skin. Results: MIF and MMP-9 were positive expression in lichen planus ,and negative or weakly positive expression in normal skin.The expressions of MIF and MMP-9 were significantly higher in lichen planus than those in the normal skin (P0.05); there were positive correlations between the expression of MIF and MMP-9 in the cases of lichen planus (r=0.636, P0.01). Conclusion: (1) MIF may be involved in the infiltration of inflammatory cells in lichen planus.(2)MMP-9 may play a role in