about of interleukin 2mrna rat anterior pituitary expression（关于白介素2的mrna鼠垂体前叶的表情）.docVIP

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about of interleukin 2mrna rat anterior pituitary expression（关于白介素2的mrna鼠垂体前叶的表情）.doc

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about of interleukin 2mrna rat anterior pituitary expression（关于白介素2的mrna鼠垂体前叶的表情）

About of interleukin 2mRNA rat anterior pituitary expression Author: Wei Gengze Zhu Yunlong Wang Gaofeng Han Xuefeng Hu Yuzhen EARTH SOCIETY CHAN Kin [Keywords] Interleukin-2 Keywords: interleukin-2; anterior pituitary; reverse transcription - polymerase chain reaction; rat Abstract: The purpose of interleukin -2 (IL-2mRNA expression in the normal rat anterior pituitary cells from primary cultures of normal rat anterior pituitary tissue and in vitro extraction of total RNA using the two-step RT -PCR technology, the corresponding size of amplified DNA fragment, this fragment was recovered and purified by gel electrophoresis and connected to the pGEM-T easy vector, and transfected into E. coli cells, and inoculated in LB medium culture 10 ~ 14h , selected positive clones for DNA sequencing. determination of the DNA results with IL-gene fragment. Thus, in the normal rat anterior pituitary tissue and cultured cells have of IL-2mRNA of expression. conclusions demonstrated for the first time that the IL -2 not only exist in the pituitary tumor and normal pituitary tissue expression. provides a theoretical basis for further study of the neuroendocrine network theory. Keywords: IL-2; anterior pituitary; RT-PCR; rats Abstract: AIM To study the expression of IL-2mRNA in rat normal anterior pituitary.METHODS Total RNA was extracted from rats’normal anterior pituitary tissues and pri-mary cultured cells in vitro.Using the two-step RT-PCR technique, we amplified a DNA fragment in comparatively the same length as that of IL-2mRNA.Through DNA a-garose gel electrophoresis, the fragment was purified and then connected with pGEM-T easy plasmid by the T4ligase.The connected plasmid was transformed into the E.coli cells. The transformed cells were planted in the Amp + LB culture medium and cultured for10 ~ 14h.The positive clones were selected and then the DNA sequencing was conducted.RE ┐ SULTS The DNA sequence was the same as that of the IL-2gene fragment . Therefore