adult meniscal cells in vitro regional culture analysis（成年半月板细胞体外地域文化分析）.docVIP

下载本文档

2
0
约1.65万字
约 16页
2017-09-05 发布于浙江
举报
版权申诉

adult meniscal cells in vitro regional culture analysis（成年半月板细胞体外地域文化分析）.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

adult meniscal cells in vitro regional culture analysis（成年半月板细胞体外地域文化分析）

Adult meniscal cells in vitro regional culture analysis [Abstract] The purpose partition meniscus cells cultured adult observation of cell growth characteristics of different regions of the meniscus, as well as the impact of the growth factor. Method sterile cut a 1-month-old male goat hind limbs meniscus D Hanks solution rinse, cut into pieces oscillation II collagenase digestion, cells were collected by centrifugation after the mesh filter. inoculated 6-well cell culture plates, each well 1 ?105, 37 ℃, the volume fraction of 0.05 of CO2, humidified culture growth curves and trypan blue staining measure cell viability. cells crawl piece line hematoxylin and eosin, and collagen type II immunohistochemical staining and RT PCR detection aggrecan expression with flow cytometry cell cycle distribution. Goat adult meniscus cells after 36 h, adherent cells of the lateral fusiform, medial area of #8203;#8203;the polygon-based, mixed cell morphology in the mixed type. The trypan blue dye test measuring cell activity of about 92% population doubling time of approximately 47.4 h. to join the human insulin-like growth factor I and basic fibroblast growth factor, cell proliferation accelerated polygonal cells increased cell crawling piece line II collagen immunohistochemical staining confirmed brown granules in the cytoplasm of transfected cells by RT PCR test results show that the purpose of bands consistent with the expected size of aggrecan detected by flow cytometry to the increase in meniscal cells in S phase after growth factor, while the G1 phase of cell reduce conclusion different regions of the meniscus cells have morphological differences. by adding growth factors to promote their growth, proliferation. Meniscus tibial insulin-like growth factor fibroblast growth factor 2 [ABSTRACT] ObjectiveTo investigate the morphology of cultured meniscal fibrochondrocytes in different areas and the influence of growth factor on the cells. MethodsThe menisci were take