经前舒颗粒对郁怒大鼠不同脑区5-HTR3B表达和5-HT含量地影响.pdfVIP

including the crossing score， rearing score and total score were markedly increase （P0.01 ）；saccharine preference index reduced （P0.01 ）. When treating with Jingqianshu graulne ，the score of open-field markedly decrease （P0.05 ） and saccharine preference index markedly increased （P0.05 ）. RT-PCR results: The mRNA expression of 5-HTR3B in different brain regions of model rats increased obviously （P0.01 ），comparing with the normal control group. When treating with Jingqianshu graulne ， the mRNA expression of 5-HTR3B decrease obviously （P0.01 ） Western-blot result: The expression of 5-HTR3B in different brain regions of model rats increased obviously （P0.01 ），comparing with the normal control group.. When treating with Jingqianshu graulne ， the expression of 5-HTR3B decrease obviously （P0.01 ） HPLC-FLU result: The contents of 5-HT in different brain regions of model rats decreased obviously （P0.01 ）， comparing with the normal control group. When treating with Jingqianshu graulne ， the contents of 5-HT increase obviously （P0.01 ）. Conclusion: We found the method by resident intruder paradigm could simulate the model of Worried and angry effectively. The expressions changes of the 5-HTR3B and the content of the 5-HT in different brain are in close relation to Worried and angry Rats. At the same time ，the Jingqianshu granule Call down-regulate the 5-HT3B expression levels and 5-HT content of model rats in different brain regions ，which maybe one of the mechanism to treat Worried and angry. Keywords: anger-in Rats, Jiangqianshu granule, 5-HT3B, RT-PCR , western-blot 目 录 引 言1 材料与方法3 1 实验材料3 1.1 实验动物3 1.2 药品与试剂3 1.3 实验仪器3 2实验步骤与方法4 2.1 模型的制备4 2.1.1大鼠的筛选及饲