a new extractive spectrophotometric method for determination of rizatriptan dosage forms using bromocresol green一种新的萃取分光光度法测定rizatriptan剂型使用溴甲酚绿.pdfVIP

Souri et al. DARU Journal of Pharmaceutical Sciences 2013, 21:12 /content/21/1/12 RESEARCH ARTICLE Open Access A New extractive spectrophotometric method for determination of rizatriptan dosage forms using bromocresol green 1* 1 2 1 Effat Souri , Abbas Kaboodari , Noushin Adib and Massoud Amanlou Abstract Background and the purpose of the study: Rizatriptan is used effectively for the treatment of migraine headache. In this study, a simple, rapid and low cost spectrophotometric method based on the ion-pair complexation is proposed for the determination of rizatriptan in raw material and dosage forms. Methods: The ion-pair complexation using bromocresol green as reagent was performed in a buffer solution and the absorbance was measured by a spectrophotometer. The ion-pair formation conditions were optimized and the accuracy and precision of the method were calculated. Results and major conclusion: Best results were achieved by using 6 ml of the bromocresol green reagent in the presence of phosphate buffer (pH 3.0). The stoichiometry of the resulted complex was 1:1. The within-day and between-day precision values were lower than 2.9 and 1.8 percent for the calibration range of 0.5–50 and 10– 100 μg/ml, respectively. The proposed method was successfully used for the determination of rizatriptan in dosage forms without any interference. Keywords: Rizatriptan, Bromocresol green, Lon-pair complexation, Spectrophotometry Introduction are based on ion-pair complexation, redox-complexation Rizatriptan benzoate (Figure 1), N, N-dimethyl-5-(1H, 1, reaction in the presence of iron ion and also oxidative 2, 4-triazol-1-ylmethyl)-1H-indole-3-ethanami