han clustering adenosine diphosphate ribose polymerase（聚腺苷二磷酸核糖聚合酶）.docVIP

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han clustering adenosine diphosphate ribose polymerase（聚腺苷二磷酸核糖聚合酶）.doc

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han clustering adenosine diphosphate ribose polymerase（聚腺苷二磷酸核糖聚合酶）

Han clustering adenosine diphosphate ribose polymerase Author: Liang Hairong, Huan-Wen Tang, Hu Dalin, ZHUANG Zhixiong [Keywords] glycosides diphosphate nuclear [Abstract] Objective: To detect human poly ADP-ribose polymerase nucleotide polymorphism in exon 1 (hPARP17 understand the Han population in Guangdong hPARP1 gene polymorphism, for the establishment of the national characteristics of DNA repair gene polymorphism data to provide basic information. methods: Select the Han population in Guangdong region of 320 basic information and blood samples extracted blood DNA by PCR amplification hPARP1 gene exon 7, using the polymerase chain reaction (PCR single-strand conformation polymorphism (SSCP) and silver staining hPARP1 gene polymorphism and DNA sequencing. Results: exon 7 outside Guangdong Hans were 320 blood specimens expansion yield material SSCP electrophoretic bands, four sample hPARP1 gene outside exon 17 SSCP electrophoresis with detection of a polymorphic bands, 12 intron of the three samples detected a polymorphic bands, the remaining six outside exon amplification products SSCP electrophoresis no polymorphic bands. exactly known sequence in the DNA sequencing results genebank normal banding pattern, 17 exon gene mutation type (T → C Conclusion: The polymorphism may exist in Guangdong Han population hPARP1 gene 17 exons and 12 introns on. [Keywords:] poly ADP-ribose polymerase -1; genetic polymorphism, polymerase chain reaction [Abstract] Objective: To investigate the nucleotide polymorphisms and distributions of seven exons in human poly (ADPribose) polymerase1 (PARP1) gene and to contribute to the construction of the special DNA repair gene polymorphisms databank. Methods: The basic materials and blood samples from 320 Guangdong health Han population were collected. The polymorphism of exon 3,11,12,13,16,17 and exon 20 of hPARP1 gene were detected by PCRSSCP, and sequencing test were performed. Results: A polymorphisms of exon 20 of PA