hantaan virus s gene 5 end of 311bp encoding prokaryotic protein expression purification and antigenicity of the（hantaan病毒s基因5 311年底bp编码原核的蛋白质表达纯化和抗原性）.docVIP

下载本文档

4
0
约1.42万字
约 14页
2017-09-06 发布于浙江
举报
版权申诉

hantaan virus s gene 5 end of 311bp encoding prokaryotic protein expression purification and antigenicity of the（hantaan病毒s基因5 311年底bp编码原核的蛋白质表达纯化和抗原性）.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

hantaan virus s gene 5 end of 311bp encoding prokaryotic protein expression purification and antigenicity of the（hantaan病毒s基因5 311年底bp编码原核的蛋白质表达纯化和抗原性）

Hantaan virus S gene 5 ‘end of 311bp encoding prokaryotic protein expression purification and antigenicity of the To write thesis net: Author: Yong Zhi-Kai Xu Yan rock Zhangfang Lin Wang Haitao Xue Xiaoping TGF - [Keywords] Hantaan virus Keywords: Hantaan virus; nucleoprotein; prokaryotic expression; purification; antigenic sites Abstract: Objective To further understand the Hantaan virus nucleoprotein amino end of antigenic characteristics. Methods build Hantaan virus S gene 5 ‘end of the 311bp fragment of the original expression vector, and induced the expression and purification of Mr. 3.6 ?104 of the fusion protein (Hantaan virus nuclear protein amino terminus Mr 1.0 ?104 and Mr 2.6 ?104 GST fusion; identified antigenic sito fes of the protein mAb, Western-blot and ELISA results only partially complete nucleoprotein mAb Mr 3.6 ?104 fusion protein reaction nucleoprotein amino terminus Mr 2.6 ?104 fragment reaction mAbs (1A8, A35, 8E8, 3A9, 3G11) can with Mr 3.6 ?104 fusion protein reaction. conclusion Hantaan virus nuclear The presence of one or several linear epitope in the protein amino-terminal Mr less than 1.0 ?104, and the carboxy terminus may be mainly plays a role in maintaining the integrity nucleoprotein dimensional conformation, and influence the identification perspective epitope of mAb with the binding of the protein. Keywords: hataan virus; nucleoprotein; prokayotic expres-sion; purification; antigentic epitope Abstract: AIM To further understand the antigen charac-teristic of the amino proximal of hantaan virus nucleoprotein.METHODS We constructed the prokaryotic vector of han-taan S gene5’-terminal311bp, then inducingly expressed and purified the Mr 3.6 ?104 fusion protein.Then , we further identified its antigentic epitopes by Western blot and ELISA methods using the nucleoprotein specific mAbs.RESULTS We detected that only parts of mAbs freacting with integrated nucleoprotein could react with Mr 3.6 ?104 fusion protein, and