hplc method for the determination of total flavonoids in ginkgo biloba soft capsule（总黄酮类化合物的高效液相色谱法测定银杏叶软胶囊）.docVIP

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2017-09-06 发布于浙江
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hplc method for the determination of total flavonoids in ginkgo biloba soft capsule（总黄酮类化合物的高效液相色谱法测定银杏叶软胶囊）.doc

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hplc method for the determination of total flavonoids in ginkgo biloba soft capsule（总黄酮类化合物的高效液相色谱法测定银杏叶软胶囊）

HPLC method for the determination of total flavonoids in Ginkgo biloba soft capsule [Abstract] Objective: by RP-HPLC determination of total flavonoids in health food Ginkgo biloba soft capsule. Methods: Agilent C18 (4.6 mm × 250 mm, 5 μm column with a mobile phase of methanol - water (with phosphoric acid pH 2.5 (50:50, flow rate of 1.0 ml / min, detection wavelength of 360 nm, the column temperature was 30 ° C. The results: quercetin, kaempferol, the isorhamnetin the linear range, respectively, from 0.050 to 0.400, 0.005 to 0.040,0.005 to 0.040 μg total flavonoids in three batches of samples were 6.466,6.455,6.838 mg / tablets Conclusion: The method is simple, accurate, and separation is good and suitable for the determination of total flavonoids in Ginkgo biloba soft capsules . [Keywords] Ginkgo biloba soft capsules, total flavonoids, quercetin, kaempferol, isorhamnetin, Determination Determination of Total Flavonoid in Yinxingye Soft Capsules by HPLC YE Xiubo1, CEN Guoying2, LI Ruiming2 (1.Traditional Chinese Medicine Hospital of Haizhu District, Guangzhou 510220, China; 2.Department of Pharmacy, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China) [Abstract] Objective: To establish an RP-HPLC method for quantitative determination of Total Flavonoid in Yinxingye Soft Capsules. Methods: The analytical column was Agilent C18 (4.6 mm × 250 mm, 5 μm) column. The mobile phase was composed of methanol and water (with phosphoric acid pH = 2) at a flow rate of 1.0 ml / min. The wavelength of UV detector was 360 nm and column temperature was 30 ℃. Results: The linear ranges of Quercetin, Kaempferol and Isorhamnetin were 0.050-0.400 , 0.005-0.040, 0.005-0.040 μg, respectively. The contents of Total Flavonoid in three batches of samples were 1 Capsule 6.466, 6.455, 6.838 mg. Conclusion: This method is sensitive, repeatable and suitable to determine the content of Total Flavonoid in Yinxingye Soft Capsules. [Keywords:]