human herpesvirus 7 u14 coding region prokaryotic expression vector（人类疱疹病毒7 u14编码区原核表达载体）.docVIP

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human herpesvirus 7 u14 coding region prokaryotic expression vector（人类疱疹病毒7 u14编码区原核表达载体）.doc

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human herpesvirus 7 u14 coding region prokaryotic expression vector（人类疱疹病毒7 u14编码区原核表达载体）

Human herpesvirus 7 U14 coding region prokaryotic expression vector : Sun Jian Ping, Xiao-Feng Wang Wang Yun Luo Bing [Keywords] exanthema [Abstract] Objective To construct the human herpes virus type 7 (HHV7) open reading frame (ORF) U14 prokaryotic expression vector, and prokaryotic expression. The major epitopes method PCR amplified HHV7 ORF U14 gene coding region (328 to 533 amino acids, respectively target gene prokaryotic expression vector pThioHis A double digested connection 1 ?TSS transformed host strain E.coli BL21 iso propyl βD thiogalactopyranoside induced fusion protein expression results gene sequence analysis showed that the target gene sequence HHV7 standard strains corresponding sequence consistent SDSPAGE electrophoresis can be observed in the relative molecular mass of 35 700 fusion protein expression by Western blot confirmed pp85 protein success expression. the conclusion HHV7 tegument protein pp85 prokaryotic expression vector construction and expression of success, and provide an experimental basis for further study of the recombinant protein-specific antibodies in HHV7. [Keywords] herpesvirus 7, the 35 700 of the envelope protein, prokaryotic expression [ABSTRACT] ObjectiveTo construct the prokaryotic expression vector of tegument protein pp85 of human herpesvirus 7 and to express it. Methods The major antigen epitopes coding sequence (328-533 aa of HHV7 ORF U14 gene was amplified by PCR and inserted into the prokaryotic expression vector pThioHis A by gene engineering technique; recombinant plasmid was transformed into E.coli BL21 and expressed by IPTG inducing. ResultsThe target gene was identical with that of HHV7 standard species, and the 35 700 fusion protein was found in SDSPAGE and proved by western blot. ConclusionRecombinant prokaryotic expression vector was gained, which is valuable for the further study on HHV7 specific antibody detection. [KEY WORDS] human herpesvirus 7; tegument protein pp85; prokaryoti