耐高三尖杉酯碱SKM-1细胞系的建立和其生物学特性研究.pdfVIP

目 录 中文 摘 要 ·······························································… … 1 英文 摘要 ·······························································… …2 前 言 ·····································································… …4 第 一 部 分 ·······························································… …6 材 料 与方法 ······················································…… ”‘6 结 果 ··································································… …8 讨 论 ··································································… …9 第 二 部分 ·······························································… … 12 材 料 与 方法 ·························································…… 12 结 果 ··································································… … 15 讨 论 ··································································… …24 全 文 小 结 ·······························································… …28 参考 文献 ·······························································……29 论 文 相 关 综述 ·························································… …34 攻 读 硕 士 学位 期 间发表 的文 章 ····································……43 综 述 一 ·······························································… …43 综 述 二 ·······························································… …52 致 谢 ·····································································… …58 中文 摘 要 耐 高三尖杉 醋碱 SK 入4一1 细胞 系 的建立 及 其 生 物 学 特 性研 究 目的 : 建立 耐 高三 尖 杉酷 碱 SK M 一1 细 胞 系 ，初 步 探 讨 该 细胞 系 的生物 学特 性 及 多 药 耐 药机 制 。 方 法 : 通 过 较 大 剂 量 间断冲 击 诱 导 法 (Pu lsati le treatm en t) 逐 渐 增 加 药 物 浓 度 的 方 法 建立 耐 高三 尖 杉 酷碱 SK M 一l 细胞 系 。光学 显微 镜 观 察 耐 药细 胞 系和 亲本 细 胞 系 的一 般 形 态 学 变 化 ，M T T 法 测 定 倍 增 时 间和 耐 药 指 数 ，绘 制 生 长 曲线 : 利 用 流 式 细胞 仪检 测 细 胞 周 期 分 布 及 细 胞 内柔 红霉 素 含 量 ; 常规 遗 传 学方 法 R 显 带 后 进 行 核 型 分 析 ;观 察 两 种 细 胞 在 裸 鼠成 瘤 的差 异 ; 多药 耐 药 相 关 基 因 (m dr一l 及 M 即 ) 及 t叩 。一n a 的表 达 采 用 R l’--p c R 方 法 检 测 。