conserved structural motifs at the c-terminus of baculovirus protein ie0 are important for its functions in transactivation and supporting hr5-mediated dna replication守恒的结构图案在杆状病毒蛋白质的糖基ie0 transactivation重要的功能,支持hr5-mediated dna复制.pdfVIP

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conserved structural motifs at the c-terminus of baculovirus protein ie0 are important for its functions in transactivation and supporting hr5-mediated dna replication守恒的结构图案在杆状病毒蛋白质的糖基ie0 transactivation重要的功能,支持hr5-mediated dna复制.pdf

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conserved structural motifs at the c-terminus of baculovirus protein ie0 are important for its functions in transactivation and supporting hr5-mediated dna replication守恒的结构图案在杆状病毒蛋白质的糖基ie0 transactivation重要的功能,支持hr5-mediated dna复制

Viruses 2012, 4, 761-776; doi:10.3390/v4050761 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Conserved Structural Motifs at the C-Terminus of Baculovirus Protein IE0 are Important for its Functions in Transactivation and Supporting hr5-mediated DNA Replication Neta Luria 1, Liqun Lu 2 and Nor Chejanovsky 1,* 1 Entomology Department, Institute of Plant Protection, The Volcani Center, POB 6, Bet Dagan 50250, Israel; E-Mail: neta@.il 2 Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; E-Mail: lqlv@ * Author to whom correspondence should be addressed; E-Mail: ninar@.il; Tel.: +972-3-9683694; Fax: +972-3-9604180. Received: 9 February 2012; in revised form: 23 April 2012 / Accepted: 27 April 2012 / Published: 4 May 2012 Abstract: IE0 and IE1 are transactivator proteins of the most studied baculovirus, the Autographa californica multiple nucleopolyhedrovirus (AcMNPV). IE0 is a 72.6 kDa protein identical to IE1 with the exception of its 54 N-terminal amino acid residues. To gain some insight about important structural motifs of IE0, we expressed the protein and C-terminal mutants of it under the control of the Drosophila heat shock promoter and studied the transactivation and replication functions of the transiently expressed proteins. IE0 was able to promote replication o