三黄汤与环丙沙星对大肠杆菌体外抑菌作用比较.docVIP

三黄汤与环丙沙星对大肠杆菌体外抑菌作用的比较 罗娜，闫俊丽，肖雄，胡思纯，何亚，蒋小平* （长沙医学院病原生物学与免疫学教研室，湖南长沙 410219） 摘要 目的：比较三黄汤与环丙沙星对耐药性大肠杆菌体外抑菌的差异，探讨两者联合使用的临床意义；方法：试管稀释法测定三黄汤、环丙沙星的MIC绘制在亚MIC浓度下两者抑菌曲线和组合用药抑菌曲线；结果：三黄汤、环丙沙星的MIC分别为450mg/ml、15.63ug/ml；在亚MIC浓度下三黄汤对大肠杆菌的抑菌曲线一直保持平稳状态；而环丙沙星抑菌曲线在起始3-4h内呈下降趋势，随后保持较平稳状态。通过20次培养后，发现三黄汤的MIC没有明显改变，而环丙沙星MIC增至90.7ug/ml，显著增高；通过不同梯度组合培养测出最佳组合浓度为三黄汤 mg/ml、环丙沙星ug/ml，最佳组合下，大肠杆菌的抑菌曲线起初表现环丙沙星的抑菌作用，但随后保持稳定组合药物中大肠杆菌对环丙沙星的药敏性有提高，MIC降至ug/ml。 关键字 三黄汤；环丙沙星；大肠杆菌 第一作者罗娜 ,女，1993-，在校本科生，生物技术专业 通讯作者：蒋小平，男，1983-，硕士研究生，主要从事微生物检验研究 Comparative inhibitory effect of SanHuang and Ciprofloxacin against E.coli Luo Na,Yan Jun-li ,Xiao Xiong,Hu Si-chun,He Ya-nan, Jiang Xiao-ping* (Department of?Pathogenic?Biology?and Immunology, Changsha Medical University, Changsha Hunan 410219) Abstract: Objective: To comparative SanHuang with ciprofloxacin reinstating E. coli in vitro antibacterial and explore the clinical significance of combination. Methods: Use tube dilution method to determine the minimum inhibitory concentration（MIC） of SanHuang and Ciprofloxacin and drawn the bacteriostatic curves when in the conditions of Sub-MIC concentration. Results: The MIC of SanHuang and Ciprofloxacin are 450mg/ml and 15.63ug/ml. When expose to Sub- MIC of SanHuang, bacteriostatic curves kept flat in the entire times, but decreased quickly in the first 3-4h and then increased to the beginning in the Ciprofloxacin liquid. After 20 times serial passage in Sub-MIC Ciprofloxacin, To combination drug culture fractional inhibitory concentration(FIC) index 0.5 for synergy ,the MIC of Ciprofloxacin to E.coli rose from 15.63ug/ml to 90.7ug/ml, while it did not increase in SanHuang. By serial dilutions, the best rate were SanHuang 112.5mg/ml and Ciprofloxacin 9.38ug/ml; bacteriostatic curves growth decreased quickly in the first 3-4h like Ciprofloxacin but soon increased to the beginning, it was found that the MIC of ciprofloxacin in combination drug dropped to 11.4ug / ml. Key Words: SanHuang, Ciprofloxacin, E. coli 随着抗生