a chitinase from aeromonas veronii cd3 with the potential to control myxozoan disease气单胞菌属的几丁质酶veronii cd3和控制myxozoan疾病的潜力.pdfVIP

A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease 1 1 2 1 1 1 3 Yuchun Liu , Zhigang Zhou *, Wei Miao , Yuting Zhang , Yanan Cao , Suxu He , Dongqing Bai , Bin Yao1* 1 Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China, 2 Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People’s Republic of China, 3 Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries Science Department, Tianjin Agricultural University, Tianjin, People’s Republic of China Abstract Background: The class Myxosporea encompasses about 2,400 species, most of which are parasites of fish and cause serious damage in aquaculture. Due to the concerns about food safety issues and limited knowledge of Myxozoa life cycle and fish immune system, no chemicals, antibiotics or immune modulators are available to control myxozoa infection. Therefore, little can be done once Myxozoa establishment has occurred. Methodology/Principal Findings: In this paper we isolated Aeromonas veronii CD3 with significant myxospore shell valve- degrading ability from pond sediment. A 3,057-bp full-length chitinase gene was consequently cloned, and the corresponding mature, recombinant chitinase (ChiCD3) produced by Escherichia coli had substantial chitinase activity. The deduced sequence of ChiCD3 contained one catalytic domain, two chitin-binding domains, and one putative signal peptide. ChiCD3 had an optimal activity at 50uC and pH 6.0, and retained more than 50% of its optimal activity under warm water aquaculture conditions (,30uC an