原创力文档a comparative study on the expression, purification and functional characterization of human adiponectin in pichia pastoris and escherichia coli表达上的比较研究,净化和功能特征的人类pastoris脂联素在毕赤酵母和大肠杆菌.pdfVIP
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a comparative study on the expression, purification and functional characterization of human adiponectin in pichia pastoris and escherichia coli表达上的比较研究,净化和功能特征的人类pastoris脂联素在毕赤酵母和大肠杆菌
Int. J. Mol. Sci. 2012, 13, 3549-3562; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
A Comparative Study on the Expression, Purification and
Functional Characterization of Human Adiponectin in Pichia
pastoris and Escherichia coli
Hussin A. Rothan 1, Ser Huy Teh 2, Kamariah Haron 3 and Zulqarnain Mohamed 2,3,*
1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603,
Kuala-Lumpur, Malaysia; E-Mail: molec.genetics@
2 Institute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala-Lumpur,
Malaysia; E-Mail: serhuy@.my
3 Center for Research in Biotechnology for Agriculture (CEBAR), University Malaya, 50603,
Kuala-Lumpur, Malaysia; E-Mail: k_haron@.my
* Author to whom correspondence should be addressed: E-Mail: zulq@.my;
Tel: +603-7967-6992; Fax: +603-7967-4957.
Received : 4 January 2012; in revised form: 28 February 2012 / Accepted: 6 March 2012 /
Published: 15 March 2012
Abstract: Adiponectin is one of the most bioactive substances secreted by adipose tissue
and is involved in the protection against metabolic syndrome, artherosclerosis and type II
diabetes. Research into the use of adiponectin as a promising drug for metabolic
syndromes requires production of this hormone in high quantities considering its molecular
isoforms. The objective of this study is to produce recombinant human a
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