a novel computational strategy to identify a-to-i rna editing sites by rna-seq data de novo detection in human spinal cord tissue新颖的计算策略识别a-to-i rna编辑网站通过rna-seq数据新创检测人类脊髓组织.pdfVIP

A Novel Computational Strategy to Identify A-to-I RNA Editing Sites by RNA-Seq Data: De Novo Detection in Human Spinal Cord Tissue 1,2 3 3 3 1,2 Ernesto Picardi , Angela Gallo , Federica Galeano , Sara Tomaselli , Graziano Pesole * ` 1 Dipartimento di Bioscienze, Biotecnologie e Scienze Farmacologiche, Universita di Bari, Bari, Italy, 2 Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle ` Ricerche, Bari, Italy, 3 RNA Editing Laboratory, Oncohaematology Department, Ospedale Pediatrico ‘‘Bambino Gesu’’, IRCCS, Rome, Italy Abstract RNA editing is a post-transcriptional process occurring in a wide range of organisms. In human brain, the A-to-I RNA editing, in which individual adenosine (A) bases in pre-mRNA are modified to yield inosine (I), is the most frequent event. Modulating gene expression, RNA editing is essential for cellular homeostasis. Indeed, its deregulation has been linked to several neurological and neurodegenerative diseases. To date, many RNA editing sites have been identified by next generation sequencing technologies employing massive transcriptome sequencing together with whole genome or exome sequencing. While genome and transcriptome reads are not always available for single individuals, RNA-Seq data are widespread through public databases and represent a relevant source of yet unexplored RNA editing sites. In this context, we propose a simple computational strategy to identify genomic positions