a novel cyclodextrin glycosyltransferase from alkaliphilic amphibacillus sp. npst-10 purification and properties一种新型环糊精糖基转移酶从alkaliphilic amphibacillus sp。npst-10净化和属性.pdfVIP

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a novel cyclodextrin glycosyltransferase from alkaliphilic amphibacillus sp. npst-10 purification and properties一种新型环糊精糖基转移酶从alkaliphilic amphibacillus sp。npst-10净化和属性.pdf

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a novel cyclodextrin glycosyltransferase from alkaliphilic amphibacillus sp. npst-10 purification and properties一种新型环糊精糖基转移酶从alkaliphilic amphibacillus sp。npst-10净化和属性

Int. J. Mol. Sci. 2012, 13, 10505-10522; doi:10.3390/ijms130810505 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A Novel Cyclodextrin Glycosyltransferase from Alkaliphilic Amphibacillus sp. NPST-10: Purification and Properties Abdelnasser S. S. Ibrahim 1,*, Ali A. Al-Salamah 1, Mohamed A. El-Tayeb 1, Yahya B. El-Badawi 1 2 and Garabed Antranikian 1 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia; E-Mails: aaasalah@ (A.A.A.-S.); mali5@.sa (M.A.E.-T.); yelbadawi@.sa (Y.B.E.-B.) 2 Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstrasse 12, 21073 Hamburg, Germany; E-Mail: antranikian@tuhh.de * Author to whom correspondence should be addressed; E-Mail: ashebl@.sa; Tel./Fax: +966-1-4675870. Received: 26 June 2012; in revised form: 5 August 2012 / Accepted: 10 August 2012 / Published: 22 August 2012 Abstract: Screening for cyclodextrin glycosyltransferase (CGTase)-producing alkaliphilic bacteria from samples collected from hyper saline soda lakes (Wadi Natrun Valley, Egypt), resulted in isolation of potent CGTase producing alkaliphilic bacterium, termed NPST-10. 16S rDNA sequence analysis identified the isolate as Amphibacillus sp. CGTase was purified to homogeneity up to 22.1 fold by starch adsorption and anion exchange