原创力文档a method for generation phage cocktail with great therapeutic potential方法一代噬菌体鸡尾酒的治疗潜力.pdfVIP
- 1、本文档共8页，可阅读全部内容。
- 2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a method for generation phage cocktail with great therapeutic potential方法一代噬菌体鸡尾酒的治疗潜力
A Method for Generation Phage Cocktail with Great
Therapeutic Potential
Jingmin Gu, Xiaohe Liu, Yue Li, Wenyu Han*, Liancheng Lei, Yongjun Yang, Honglei Zhao, Yu Gao,
Jun Song, Rong Lu, Changjiang Sun, Xin Feng
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People’s Republic of China
Abstract
Background: Bacteriophage could be an alternative to conventional antibiotic therapy against multidrug-resistant bacteria.
However, the emergence of resistant variants after phage treatment limited its therapeutic application.
Methodology/Principal Findings: In this study, an approach, named ‘‘Step-by-Step’’ (SBS), has been established. This
method takes advantage of the occurrence of phage-resistant bacteria variants and ensures that phages lytic for wild-type
strain and its phage-resistant variants are selected. A phage cocktail lytic for Klebsiella pneumoniae was established by the
SBS method. This phage cocktail consisted of three phages (GH-K1, GH-K2 and GH-K3) which have different but overlapping
host strains. Several phage-resistant variants of Klebsiella pneumoniae were isolated after different phages treatments. The
virulence of these variants was much weaker [minimal lethal doses (MLD).1.36109 cfu/mouse] than that of wild-type K7
countpart (MLD = 2.5 6103 cfu/mouse). Compared with any single phage, the phage cocktail significantly reduced the
mutation frequency of Klebsiella pneumoniae and effectively rescued Klebsiella pneumoniae bacteremia in a murine K7 strain
challenge model. The minimal protective dose (MPD) of the phage cocktail which was sufficient to protect bacteremic mice
4
from lethal K7 infection was only 3.0 610 pfu, significantly smaller (p,0.01) than that of single monophage. Moreover, a
delayed administration of this phage cock
您可能关注的文档
- a combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of micrornas and their targets in cucumber高通量测序和degradome分析揭示了一个综合的方法组织特定表达的小分子核糖核酸和黄瓜的目标.pdf
- a combination of dopamine genes predicts success by professional wall street traders结合多巴胺基因预测成功到专业的华尔街交易员.pdf
- a combined epigenetic and non-genetic approach for reprogramming human somatic cells结合表观遗传和非遗传的方式为人类体细胞重编程.pdf
- a combined screening platform for hiv treatment failure and resistance联合筛查艾滋病毒治疗失败和阻力的平台.pdf
- a combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences结合转录组和lipidomics分析皮下,附睾的和肠系膜脂肪组织显示明显的功能差异.pdf
- a comment on the relation between diffraction and entropy一个衍射和熵之间的关系发表评论.pdf
- a common anterior insula representation of disgust observation, experience and imagination shows divergent functional connectivity pathways常见的脑前叶表示厌恶的观察、经验和想象力显示不同的功能连通性通路.pdf
- a common bace1 polymorphism is a risk factor for sporadic creutzfeldt-jakob disease一个常见的bace1多态性是零星的克雅氏病的一个危险因素.pdf
- a common ca2+-driven interdomain module governs eukaryotic ncx regulation常见的ca2 +简况interdomain模块控制真核ncx的监管.pdf
- a common cnr1 (cannabinoid receptor 1) haplotype attenuates the decrease in hdl cholesterol that typically accompanies weight gain共同cnr1(大麻素受体1)单体型变弱高密度脂蛋白胆固醇的降低,通常也伴随着体重增加.pdf
- a method for rapid and simultaneous mapping of genetic loci and introgression sizes in nematode species方法快速、同步映射在线虫物种基因位点和基因渗入的大小.pdf
- a method to compute multiplicity corrected confidence intervals for odds ratios and other relative effect estimates一个方法来计算多重性校正优势比的置信区间估计和其他相对影响.pdf
- a method for quantitative determination of biofilm viability方法定量测定生物膜的可行性.pdf
- a method for the automated, reliable retrieval of publication-citation records方法自动、可靠检索publication-citation记录.pdf
- a method for removal of low frequency components associated with head movements from dual-axis swallowing accelerometry signals方法去除低频组件从双轴吞下个加速器与头部运动信号.pdf
- a methodological framework for evaluating the evidence for complementary and alternative medicine (cam) for cancer方法论的框架来评估的证据补充和替代医学(cam)癌症.pdf
- a method to use solar energy for the production of gas from marine hydrate-bearing sediments a case study on the shenhu area使用太阳能的方法生产的气体从海洋沉积物hydrate-bearing shenhu地区案例研究.pdf
- a method to model season of birth as a surrogate environmental risk factor for disease方法模型的季节出生作为一个代理环境危险因素疾病.pdf
- a metagenomic analysis of pandemic influenza a (2009 h1n1) infection in patients from north america2009年宏基因组分析甲型流感(h1n1)感染患者从北美.pdf
- a methodological approach to assessing the health impact of environmental chemical mixtures pcbs and hypertension in the national health and nutrition examination survey方法论的方法来评估环境健康影响的化学混合物多氯联苯和高血压在全国健康和营养调查.pdf
文档评论（0）