a theoretical analysis of the feasibility of a singularity-induced micro-electroporation system理论分析的可行性singularity-induced micro-electroporation系统.pdfVIP

A Theoretical Analysis of the Feasibility of a Singularity- Induced Micro-Electroporation System 1 2 Gregory D. Troszak *, Boris Rubinsky 1 Department of Mechanical Engineering, University of California, Berkeley, California, United States of America, 2 Graduate Program in Biophysics and Department of Mechanical Engineering, University of California, Berkeley, California, United States of America Abstract Electroporation, the permeabilization of the cell membrane lipid bilayer due to a pulsed electric field, has important implications in the biotechnology, medicine, and food industries. Traditional macro and micro-electroporation devices have facing electrodes, and require significant potential differences to induce electroporation. The goal of this theoretical study is to investigate the feasibility of singularity-induced micro-electroporation; an electroporation configuration aimed at minimizing the potential differences required to induce electroporation by separating adjacent electrodes with a nanometer-scale insulator. In particular, this study aims to understand the effect of (1) insulator thickness and (2) electrode kinetics on electric field distributions in the singularity-induced micro-electroporation configuration. A non-dimensional primary current distribution model of the micro-electroporation channel shows that while increasing insulator thickness results in smaller electric field magnitudes, electroporation can still be performed with insulators thick enough to be made with microfabrication techniques. Furthermore, a secondary current distribution model of the singularity-induced micro- electroporation configuration with inert platinum electrodes and water electrolyte indicates that electrode kin