反复性脑缺血海马钙积聚与神经元损害.docVIP

反复性脑缺血海马钙积聚与神经元损害 曹仁存 王庆松 林杭 吴渝宪 王伟文(成都军区总医院神经内科,成都610083) [摘要] 目的 研究反复性脑缺血海马神经元的病理损害及其机制。方法 应用45Ca放射自显影及光镜对比观察大鼠单次性和反复性脑缺血及NMDA受体拮抗剂MK-801治疗后海马钙积聚和神经元损害的病理改变。结果 反复缺血组海马异常钙积聚与迟发性神经元坏死（DND）明显重于单次缺血组(P0.01)；MK-801显著减轻海马钙积聚与组织病理损害(P0.01)。结论 反复非致死性短暂脑缺血导致海马神经元显著累积性损害与DND，兴奋性氨基酸(EAA)及Ca2+可能起着重要作用。 【关键词】 脑缺血; 反复缺血; 海马; 钙积聚；兴奋性氨基酸（EAA） Calcium accumulation and neuronal damage in the rat hippocampus following repeated cerebral ischemia CAO Ren-cun, WANG Qing-song, LIN Hang, WU Yu-xian, WANG Wei-wen （Departerment of Neurology, Chengdu Military General Hospital, Chendu 610083, China） 【Abstract】　Objective 　To explore the neuronal pathological damage and its mechanism of hippocampus following repeated cereebral ischemia. Methods 45Ca autoradiography and light microscopy were used to observe the calcium accumulation and neuronal pathological changes of hippocampus following repeated cerbral ischemia in the rats in comparison with the damage following single cerebral ischemia. The effects of MK-801, a NMDA receptor antagonist, were also examined. Results The abnormal calcium accumulation and delayed neuronal death (DND) were found to be more server in the hippocampus in the group of repeated cerebral ischemia than those in the single ischemia insult group(P0.01). MK-801 is effective in reducing or ameliorating the calcium accumulation and histopathologiucal damage in the hippocampus(P0.01). Conclusion Repeated nonlethal cerebral ischemia resulted in a more maked cumulative damage and DND in the hippocampus, the excitatory amino acid(EAA) and ca2+ may play a major role in it. . 【Key words】 Cerebral ischemia; Repeated ischemia; Hippocampus; Calcium accumulation; Excitory amino acid (EAA) 反复非致死短暂脑缺血导致易损神经元显著累积性损害，进而发生多灶性脑梗死、血管性痴呆（vascular dementia, VD），正为当代临床所关注，然至今其确切的病理损害型式和机制尚不明了[1-2]。海马是学习记忆的重要结构，同时亦为脑缺血选择性易损区[3]，并常因此而导致或加重VD的发生发展。本实验应用45Ca放射自显影，结合组织病理学，动态对比观察大鼠单次性和反复性脑缺血海马神经元的病理损害及N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801的保护作用，以探讨反复性脑缺血海马神经元损伤的病理特点和机制，为临床防治提