维素降解菌的分离及酶活研究.doc

东北农业大学学士学位论文 学号：A 纤维素降解菌的分离及酶活研究 学生姓名： 指导教师： 所在院系：学院 所学专业： 研究方向： 东 北 农 业 大 学 中国·哈尔滨 201年5月Bachelors Degree Thesis of Northeast Agricultural University Student Number: A Separating on the Cellulose-decomposing Bacteria and Study on Its Enzyme Activity Student: Wang Bing Instructor: Huang Xiaomei College: Resources Environment College Specialty: Applied biological Science Research Direction: Microorganism Northeast Agricultural University China · Harbin 2013-5摘 要 关键词；；； Abstract Cellulose, the most abundant carbohydrate in nature, has proportion that is more than the sum of other carbohydrates.and it is a kind of important renewable resources and energy which has not been making full use of. It is also the largest class of environmental pollutants and one of the main sources of COD and SS in papermaking waste-water. Cellulose, as a kind of sustainable energy material, its development has aroused great attention. Transform decomposed cellulose into animals’ easily absorbed or used energy, food, feed or chemical raw materials, is an important way of promoting the rational utilization of cellulose. Therefore screening of cellulose-decomposing bacteria and measuring its enzyme activity has the vital significance. This study was designed to sample from soil under different environment, separated the strain by cellulose agar selection medium ,preliminary confirmed its ability to decompose cellulose by Congo red staining. Then each selected one strain from strains which were isolated from different soil samples. Inoculating each of the screened strain on collapse of the medium filter paper culture medium, degradation extent and speed of filter paper was observed and compared. Preparing the screened bacteria suspension and inoculating in liquid medium, enzyme activation of CMC. Experimental results show that: soil in different environment both has cellulose-decomposing bacteria. Judging by Congo red plate hydrolysi