recapitulation of fibromatosis nodule by multipotential stem cells in immunodeficient mice重演的纤维瘤病结节multipotential在免疫缺陷小鼠干细胞.pdfVIP

Recapitulation of Fibromatosis Nodule by Multipotential Stem Cells in Immunodeficient Mice 1,3,4 5 3,4 6 3,4 Jung-Pan Wang , Yun-Ju Hui , Shih-Tien Wang , Hsiang-Hsuan Michael Yu , Yi-Chao Huang , En- Rung Chiang1,4, Chien-Lin Liu3,4, Tain-Hsiung Chen3,4, Shih-Chieh Hung1,2,4,5* 1 Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, 2 Department of Pharmacology, School of Medicine, National Yang- Ming University, Taipei, Taiwan, 3 Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan, 4 Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan, 5 Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, 6 Department of Radiation Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, United States of America Abstract Musculoskeletal fibromatosis remains a disease of unknown etiology. Surgical excision is the standard of care, but the recurrence rate remains high. Superficial fibromatosis typically presents as subcutaneous nodules caused by rapid myofibroblast proliferation followed by slow involution to dense acellular fibrosis. In this study, we demonstrate that fibromatosis stem cells (FSCs) can be isolated from palmar nodules but not from cord or normal palm tissues. We found that FSCs express surface markers such as CD29, CD44, CD73, CD90, CD105, and CD166 but do not express CD34, CD45, or CD133. We also found that FSCs are capable of expanding up to 20 passages, that these cells include myofibroblasts, osteob