regulation of granulocyte and macrophage populations of murine bone marrow cells by g-csf and cd137 protein监管粒细胞和巨噬细胞的小鼠骨髓细胞的数量由g - csf和cd137蛋白质.pdfVIP

Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein 1 1,2 Dongsheng Jiang , Herbert Schwarz * 1 Department of Physiology, and Immunology Programme, Yong Loo Lin School of Medicine, Singapore, Singapore, 2 NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore Abstract Background: Granulocytes and monocytes/macrophages differentiate from common myeloid progenitor cells. Granulocyte colony-stimulating factor (G-CSF) and CD137 (4-1BB, TNFRSF9) are growth and differentiation factors that induce granulocyte and macrophage survival and differentiation, respectively. This study describes the influence of G-CSF and recombinant CD137-Fc protein on myelopoiesis. Methodology/Principal Findings: Both, G-CSF and CD137 protein support proliferation and survival of murine bone marrow cells. G-CSF enhances granulocyte numbers while CD137 protein enhances macrophage numbers. Both growth factors together give rise to more cells than each factor alone. Titration of G-CSF and CD137 protein dose-dependently changes the granulocyte/macrophage ratio in bone marrow cells. Both factors individually induce proliferation of hematopoietic progenitor cells (lin- + , c-kit ) and differentiation to granulocytes and macrophages, respectively. The combination of G-CSF and CD137 protein further increases proliferation, and results in a higher number of macrophages than CD137 protein alone, and a lower number of granulocytes than G-CSF alone demonstrating that CD137 protein-induced monocytic differentiation is dominant over G-CSF-induced granulocytic differentiation. CD137 protein