relation of dna methylation of 5′-cpg island of acsl3 to transplacental exposure to airborne polycyclic aromatic hydrocarbons and childhood asthmadna甲基化的关系5u2032cpg岛acsl3经胎盘的接触机载多环芳烃和儿童哮喘.pdfVIP

Relation of DNA Methylation of 59-CpG Island of ACSL3 to Transplacental Exposure to Airborne Polycyclic Aromatic Hydrocarbons and Childhood Asthma 1. 2. 1 1 2 1,5 Frederica Perera , Wan-yee Tang , Julie Herbstman , Deliang Tang , Linda Levin , Rachel Miller , Shuk-mei Ho2,3,4* 1The Columbia Center for Children’s Environmental Health, Columbia University Mailman School of Public Health, New York, New York, United States of America, 2 Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, United States of America, 3 Center for Environmental Genetics, University of Cincinnati, Cincinnati, Ohio, United States of America, 4 Cancer Center, College of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of America, 5 Division of Pulmonary, Allergy and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York, United States of America Abstract In a longitudinal cohort of ,700 children in New York City, the prevalence of asthma (.25%) is among the highest in the US. This high risk may in part be caused by transplacental exposure to traffic-related polycyclic aromatic hydrocarbons (PAHs) but biomarkers informative of PAH-asthma relationships is lacking. We here hypothesized that epigenetic marks associated with transplacental PAH exposure and/or childhood asthma risk could be identified in fetal tissues. Mothers completed personal prenatal air monitoring for PAH exposure determination. Methylation sensitive restriction fingerprinting was used to analyze umbilical cord white blood cell (UCWBC) DNA of