resolving the role of actoymyosin contractility in cell microrheology解决在细胞microrheology actoymyosin收缩的作用.pdfVIP

Resolving the Role of Actoymyosin Contractility in Cell Microrheology 1 2,3 1,3 Christopher M. Hale , Sean X. Sun , Denis Wirtz * 1 Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America, 2 Department of Mechanical Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America, 3 Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, Maryland, United States of America Abstract Einstein’s original description of Brownian motion established a direct relationship between thermally-excited random forces and the transport properties of a submicron particle in a viscous liquid. Recent work based on reconstituted actin filament networks suggests that nonthermal forces driven by the motor protein myosin II can induce large non-equilibrium fluctuations that dominate the motion of particles in cytoskeletal networks. Here, using high-resolution particle tracking, we find that thermal forces, not myosin-induced fluctuating forces, drive the motion of submicron particles embedded in the cytoskeleton of living cells. These results resolve the roles of myosin II and contractile actomyosin structures in the motion of nanoparticles lodged in the cytoplasm, reveal the biphasic mechanical architecture of adherent cells—stiff contractile stress fibers interdigitating in a network at the cell cortex and a soft actin meshwork in the body of the cell, validate the method of particle tracking-microrheology, and reconcile seemingly disparate atomic force microscopy (AFM) and particle- tracking microrheology measurements of living cells. Citation: Hale CM, Sun SX, Wirtz D (2009) Resolving the Role of Actoymyosin Contractility in Cell Microrheology. PLoS ONE