response of archaeal communities in the rhizosphere of maize and soybean to elevated atmospheric co2 concentrations响应的热点地区的玉米和大豆根际大气中的二氧化碳浓度升高.pdfVIP
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response of archaeal communities in the rhizosphere of maize and soybean to elevated atmospheric co2 concentrations响应的热点地区的玉米和大豆根际大气中的二氧化碳浓度升高
Response of Archaeal Communities in the Rhizosphere of
Maize and Soybean to Elevated Atmospheric CO2
Concentrations
David M. Nelson1,2*, Isaac K. O. Cann2,3,4, Roderick I. Mackie2,3
1 Appalachian Laboratory, University of Maryland Center for Environmental Science, Frostburg, Maryland, United States of America, 2 Institute for Genomic Biology,
University of Illinois, Urbana, Illinois, United States of America, 3 Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America,
4 Department of Microbiology, University of Illinois, Urbana, Illinois, United States of America
Abstract
Background: Archaea are important to the carbon and nitrogen cycles, but it remains uncertain how rising atmospheric
carbon dioxide concentrations ([CO2]) will influence the structure and function of soil archaeal communities.
Methodology/Principal Findings: We measured abundances of archaeal and bacterial 16S rRNA and amoA genes,
phylogenies of archaeal 16S rRNA and amoA genes, concentrations of KCl-extractable soil ammonium and nitrite, and
potential ammonia oxidation rates in rhizosphere soil samples from maize and soybean exposed to ambient (,385 ppm)
and elevated (550 ppm) [CO ] in a replicated and field-based study. There was no influence of elevated [CO ] on copy
2 2
numbers of archaeal or bacterial 16S rRNA or amoA genes, archaeal community composition, KCl-extractable soil
ammonium or nitrite, or potential ammonia oxidation rates for samples from maize, a model C4 plant. Phylogenetic
evidence indicated decreased relative abundance of crena
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