respiratory chain complexes in dynamic mitochondria display a patchy distribution in life cells线粒体呼吸链复合物在动态显示在生活细胞的分布.pdfVIP

Respiratory Chain Complexes in Dynamic Mitochondria Display a Patchy Distribution in Life Cells 1 1,2 3 3 4 4 Britta Muster , Wladislaw Kohl , Ilka Wittig , Valentina Strecker , Friederike Joos , Winfried Haase , ¨ 1, Karin Busch1,2* Jurgen Bereiter-Hahn 1 Institute of Kinematic Cell Research, Department of Biology, University of Frankfurt, Frankfurt/Main, Germany, 2 Laboratory for Mitochondrial Dynamics, Department of Biology, University of Osnabrueck, Osnabrueck, Germany, 3 Institute of Molecular Bioenergetics, Medical School, University of Frankfurt, Frankfurt/Main, Germany, 4 Electron Facility, Max Planck Institute of Biophysics, Frankfurt/Main, Germany Abstract Background: Mitochondria, the main suppliers of cellular energy, are dynamic organelles that fuse and divide frequently. Constraining these processes impairs mitochondrial is closely linked to certain neurodegenerative diseases. It is proposed that functional mitochondrial dynamics allows the exchange of compounds thereby providing a rescue mechanism. Methodology/Principal Findings: The question discussed in this paper is whether fusion and fission of mitochondria in different cell lines result in re-localization of respiratory chain (RC) complexes and of the ATP synthase. This was addressed by fusing cells containing mitochondria with respiratory complexes labelled with different fluorescent proteins and resolving their time dependent re-localization in living cells. We found a complete reshuffling of RC complexes throughout the entire chondriome in single HeLa cells within 2–3 h by organelle fusion and fission. Polykaryons of fused ce