response to arsenate treatment in schizosaccharomyces pombe and the role of its arsenate reductase activity砷酸反应治疗在粟酒裂殖酵母和其砷酸还原酶活性的作用.pdfVIP

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2017-09-09 发布于上海
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response to arsenate treatment in schizosaccharomyces pombe and the role of its arsenate reductase activity砷酸反应治疗在粟酒裂殖酵母和其砷酸还原酶活性的作用.pdf

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response to arsenate treatment in schizosaccharomyces pombe and the role of its arsenate reductase activity砷酸反应治疗在粟酒裂殖酵母和其砷酸还原酶活性的作用

Response to Arsenate Treatment in Schizosaccharomyces pombe and the Role of Its Arsenate Reductase Activity 1. ´ 2. ´ 1 1 Alejandro Salgado , Ana Lopez-Serrano Oliver , Ana M. Matia-Gonzalez , Jael Sotelo , Sonia Zarco- ´ 2 ˜ 2 ´ 2 ´ 1 Fernandez , Riansares Munoz-Olivas , Carmen Camara , Miguel A. Rodrıguez-Gabriel * ´ ´ ´ 1 Centro de Biologıa Molecular ‘‘Severo Ochoa’’, Universidad Autonoma de Madrid-Consejo Superior de Investigaciones Cientıficas, Madrid, Spain, 2 Departamento de ´ ´ ´ Quımica Analıtica, Facultad de CC. Quımicas, Universidad Complutense de Madrid, Madrid, Spain Abstract Arsenic toxicity has been studied for a long time due to its effects in humans. Although epidemiological studies have demonstrated multiple effects in human physiology, there are many open questions about the cellular targets and the mechanisms of response to arsenic. Using the fission yeast Schizosaccharomyces pombe as model system, we have been able to demonstrate a strong activation of the MAPK Spc1/Sty1 in response to arsenate. This activation is dependent on Wis1 activation and Pyp2 phosphatase inactivation. Using arsenic speciation analysis we have also demonstrated the previously unknown capacity of S. pombe cells to reduce As (V) to As (III). Genetic analysis of several fission yeast mutants point towards the cell cycle